1. Applications
UV–Vis spectrophotometers are widely used in:
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Quantitative analysis (Beer–Lambert Law)
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Pharmaceutical analysis & drug identification
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DNA/RNA & protein estimation
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Environmental testing (water quality)
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Food & beverage quality control
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Chemical kinetics & reaction monitoring
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Academic and industrial research
2. Compliance Standards
Typical compliance and regulatory standards:
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USP (United States Pharmacopeia)
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EP (European Pharmacopeia)
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JP (Japanese Pharmacopeia)
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ISO/IEC 17025 (Calibration standards)
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21 CFR Part 11 (Data integrity, optional software)
Key performance parameters defined:
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Wavelength accuracy
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Photometric accuracy
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Repeatability
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Stray light & resolution
3. Product Description
A UV–Vis scanning spectrophotometer is an analytical instrument that:
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Measures absorbance or transmittance of light in UV (190–400 nm) and visible (400–700 nm) regions
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Uses light sources (Deuterium for UV, Tungsten/Halogen for visible)
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Scans across wavelengths to produce spectra of samples
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Determines concentration based on light absorption characteristics
4. Technical Specifications (Typical)
|
Parameter |
Specification |
|
Optical System |
Double beam / Single beam |
|
Wavelength Range |
190 – 900 nm (or wider) |
|
Spectral Bandwidth |
1 nm or better |
|
Wavelength Accuracy |
±0.1 to ±1 nm |
|
Photometric Range |
-0.3 to 3 Abs |
|
Photometric Accuracy |
±0.003 Abs |
|
Stray Light |
0.01% |
|
Light Source |
Deuterium & Tungsten/Halogen lamps |
|
Detector |
Photodiode / PMT |
|
Scan Speed |
Fast scanning (full scan in seconds) |
|
Display |
LCD / PC software control |
|
Data Output |
USB / LAN / Software |
(Values based on standard tender/lab-grade instruments)
5. Apparatus is Supplied With
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Main spectrophotometer unit
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Quartz & glass cuvettes
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Dust cover
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Power cable
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User manual
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Software (if PC-controlled)
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Calibration certificate
6. Common Capacities Available
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Standard cuvette size: 1 cm path length
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Microvolume (1–2 µL for advanced models)
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Multi-cell holders (4/6/8 cells)
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Sample volumes:
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Micro: 1–100 µL
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Standard: 1–5 mL
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7. Optional Accessories
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Auto sampler
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Temperature-controlled cell holder
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Sipper system (flow-through)
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Integrating sphere (for solids/powders)
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Printer & PC software
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Validation kits (IQ/OQ/PQ)
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Fiber optic probes
8. Dimensions & Weight (Approx.)
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Dimensions: 450 × 350 × 200 mm (varies by model)
Weight: 10 – 25 kg